Mahiru Kouzuki, a skilled artist, gives up on her passion after her elementary school classmates ridicule her colorful jellyfish mural. Several years later, upon encountering an unexpected admirer in Kano Yamanouchi—a former idol with a troubled past—Mahiru decides to pursue her childhood dreams once more.
Tasked with designing the mascot for Kano's new music project, JELEE, Mahiru enlists the help of her childhood friend and professional streamer Kiui Watase to make JELEE's first music video. Together with Kim Anouk Mei Takanashi, a pianist and fan of Kano's work as an idol, the girls aim to turn JELEE into a global hit. However, if they want to succeed in this ambitious endeavor, they will first have to free
themselves from the shackles of their pasts.
So, Kiui Watase is AFAB but they use male or NB character as vtuber model to stream. It's a bit unclear if Kiui is transmasc or non-binary, the show never said what pronouns they prefer, so that's why i use they/them. Kiui doesn't like that people judge them by their appearance, it shows thorought out the anime. There is one scene that is especially memorable, it's culmination of Kiui's story, they said on public how they feel about their identity, it's a short one but very powerful.
The anime isn't primary focused on Kiui or their gender identity but it's part of the story, you won't have the same story if you remove Kiui identity, I think it's good representation.
P.S. originally anime aired with 12 episodes, the 13 episode came out later separetely, I watched all of them, so what i said is related to all 13 episodes.